Other

Part:BBa_K4374007:Design

Designed by: Sarah García Hualde   Group: iGEM22_Navarra_BG   (2022-08-26)


CSY4 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 502
    Illegal AgeI site found at 573
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part supports 3A Assembly method. A mutation in position 351 was generated to eliminate XbaI site (T into C). A mutation in position 874 was generated to eliminate EcoRI site (G into C). A mutation in position 886 was generated to eliminate EcoRI site (G into C).


Source

GenBank At2g44350


References